TABLE 4.
Frequency and molecular diversity of the G. duodenalis, Cryptosporidium spp. and E. bieneusi sequences successfully genotyped in the canine population investigated in the region of Madrid (Spain). GenBank accession numbers are provided.
| Species | Genotype | Sub‐genotype | Locus | No. isolates | Reference sequence | Stretch | Single nucleotide polymorphisms | GenBank ID |
|---|---|---|---|---|---|---|---|---|
| Giardia duodenalis | A | – | ssu rRNA | 1 | M54878 | 51−289 | G113T | OQ722809 |
| C | – | ssu rRNA | 1 | AF199449 | 14−290 | A199R | OQ722810 | |
| D | – | ssu rRNA | 1 | AF199443 | 70−291 | None | OQ722811 | |
| A | AI | gdh | 1 | L40509 | 64−491 | C447Y | OQ679953 | |
| C | – | gdh | 2 | U60984 | 76−496 | None | OQ679954 | |
| C | – | gdh | 1 | U60984 | 76−491 | A212T | OQ679955 | |
| D | – | gdh | 1 | U60986 | 67−491 | None | OQ679956 | |
| D | – | gdh | 1 | U60986 | 73−491 | T240C, T429C, G441A | OQ679957 | |
| D | – | gdh | 1 | U60986 | 64−423 | T240C, T429C, G441A, T459A | OQ679958 | |
| D | – | gdh | 1 | U60986 | 125−417 | T312Y, C375Y | OQ679959 | |
| D | – | gdh | 1 | U60986 | 76−496 | C375Y, T429Y, G441R | OQ679960 | |
| A | AI | bg | 1 | AY655702 | 33−523 | None | OQ679961 | |
| D | – | bg | 1 | AY545647 | 98−596 | None | OQ679962 | |
| D | – | bg | 1 | AY545647 | 98−590 | A159G, A201G, T251Y | OQ679963 | |
| D | – | bg | 2 | AY545647 | 102−555 | A201G | OQ679964 | |
| D | – | bg | 1 | AY545647 | 102−590 | A201G, C207Y | OQ679965 | |
| C+D | – | bg | 1 | AY545647 | 96−594 | T123Y, T132Y, T150Y, A159W, T165K, T177Y, G183S, A194R, A201G, A202R, C207S, A231M, T243Y, T255Y, G273R, T276Y, A282M, A291R, C309Y, A312M, G327S, A387R, T390Y, T426Y, T441Y, G496R, T510Y, T513Y, T519Y, A552R, A570R, A573C, T579K | OQ679966 | |
| C | – | tpi | 1 | AY228641 | 21−532 | G37A, C63T, G136T, T316C, C369T, A379C | OQ679967 | |
| C | – | tpi | 1 | AY228641 | 19−531 | C49Y, G136K, T316Y, A368R, C369Y, A379M | OQ679968 | |
| Cryptosporidium parvum | – | – | ssu rRNA | 1 | AF112571 | 528−1030 | A646G, T649G, 686_689DelTAAT, T693A, T709C | OQ722812 |
| Cryptosporidium canis | – | – | ssu rRNA | 4 | AF112576 | 538−1021 | None | OQ722813 |
| – | XXi1a | gp60 | 1 | – | – | – | OQ679969 | |
| Enterocytozoon bieneusi | – | PtEb IX | ITS | 1 | AF059610 | 34−416 | None | OQ722933 |
bg, β‐giardin; Del, deletion; gdh, glutamate dehydrogenase; ITS, internal transcribed spacer; K, T/G; M, C/A; R, A/G; S, G/C; ssu rRNA, small subunit ribosomal RNA; tpi, triose phosphate isomerase; Y: C/T.