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. 2023 Oct 24;24(21):15534. doi: 10.3390/ijms242115534

Figure 7.

Figure 7

Validation results of common and specific cold-responsive candidate genes in pig longissimus dorsi muscle tissue and satellite cells. (A) qRT-PCR verification of candidate genes in the longissimus dorsi muscle tissue of LW and MS pigs at 25 °C and 4 °C. The left y-axis and blue bars represent the relative expression levels of eight candidate genes in qRT-PCR, and the right y-axis and red bars represent the FPKM values of eight candidate genes obtained from RNA sequencing. (B) Western blot verification of candidate genes in the longissimus dorsi muscle tissue of LW and MS pigs at 25 °C and 4 °C. (C) qRT-PCR verification of candidate genes in porcine skeletal muscle satellite cells at 37 °C and 32 °C. (D) Detection of interference efficiency of PRSS8 in porcine skeletal muscle satellite cells. (E) Effects of PRSS8 interference on mRNA levels of heat production and energy metabolism in porcine skeletal muscle satellite cells. (F) Effects of PRSS8 interference on protein levels of heat production and energy metabolism in porcine skeletal muscle satellite cells. p values were determined using the two-tailed unpaired Student’s t-test. ** p < 0.01; * p < 0.05. LW and MS denote Large White pigs and Mashen pigs, respectively; 25 and 4 represent 25 °C and 4 °C, respectively.