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. 2023 Oct 29;12(21):3715. doi: 10.3390/plants12213715

Figure 5.

Figure 5

Regulation of OsCTPS1 protein stability. (A) Effect of treatment with mock or different signalling inhibitors on the stability of OsCTPS1-sGFP protein. Protoplasts were isolated from the OsCTPS1-sGFP OX transgenic plants and then incubated with 30 μM MG132, 2 μM staurosporine, 2 μM okadaic acid, 100 μM E64d, and 116.86 μM 6-diazo-5-oxo-L-norleucine (DON). After treatments, GFP fluorescence intensity was measured every 30 min for 15 h; n = 3. (B) MapMan overviews of regulation about differentially accumulated proteins in WT and osctps1-2 in 1 DAP seeds.