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Auraptene induces AMPK activation in HEK-293 and PC3 cells but not in DU145 cells. (a,c,e) AMPKα, phosphor-AMPKα, ACC, phosphor-ACC, and actin were detected using immunoblotting after treatment with auraptene at the indicated concentrations for 8 h. AICAR (1 mM) was employed as a positive control. (a) HEK-293, (c) PC3, and (e) DU145. (b,d,f) Cells were treated with 30 μM auraptene for 1, 2, 4, 8, or 24 h, and immunoblotting was then performed. (b) HEK-293, (d) PC3, and (f) DU145.
