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. 2023 Nov 6;24(21):16011. doi: 10.3390/ijms242116011

Figure 4.

Figure 4

Auraptene attenuates the expressions of AR and PSA in LNCaP cells. (a) Protein levels of AR and PSA in LNCaP cells were examined using immunoblotting after treatment with 0, 3, 10, or 30 μM auraptene for 24 h. (b,c) Relative band intensities of (b) AR and (c) PSA protein levels are shown as bar graphs (n = 3). (d) Immunoblotting analysis of AR and PSA in LNCaP cells after treatment with 1 mM AICAR for 8 or 24 h. (e) LNCaP cells were treated with auraptene at 30 μM for 6 h, and the mRNAs of AR, PSA, and FKBP5 were examined using quantitative real-time PCR analysis. (n = 4–6). (* p < 0.05, ** p < 0.01, *** p < 0.001).