Figure 5.

Auraptene-induced downregulation of PSA was partially reversed by suppressing AMPK. (a) Effects of the treatment with AMPK siRNA on the protein levels of AR and PSA. LNCaP cells were transfected with control siRNA or AMPK siRNA for 72 h and then treated with 10 μM of auraptene (or vehicle as a control) for 24 h. PSA, AR, AMPK, phosphor-AMPKα, ACC, phosphor-ACC, and actin were detected using immunoblotting. (b) Quantification of relative band intensities (PSA/actin) (n = 4). (c) Effects of compound C on the protein levels of AR and PSA. LNCaP cells were pre-treated with 5 μM of compound C for 1 h and then treated with 0, 3, or 10 μM of auraptene for 24 h. (* p < 0.05, *** p < 0.001).