Figure 1.

Timeline of important events in liver organoid technology. Rat liver cells successfully formed spheroidal aggregates in non-adherent plastic substratum [5]. Hepatocytes and fibroblasts co-culture formed 3D structures [6]. Biodegradable hydrogels used to generate liver organoids in vitro [7]. Liver parenchymal and non-parenchymal cells isolated and co-cultured to form organoids [8]. 3D Cell and biomaterial complex formation [9]. Development of bioreactor for the generation of liver organoids using bio-artificial liver [10]. Generation of vascularized liver organoids [11]. Generation of organoids from patient-derived cells [12]. Generation of liver ductal organoids to recapitulate SAR-CoV-2 infection [13]. Development of fetal liver organoids for gene editing using CRISPR-Cas9 [14].