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. 2023 Nov 10;12(11):bio059758. doi: 10.1242/bio.059758

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — The four steps required to process data using PyCalibrate. After navigating to the website (Ai) and registering an account (Aii), the user can create projects (B) in which to collect image stacks of point spread functions. Once an image stack has been uploaded (C) the user can either download the raw data (Ciii) or process it (Civ). After processing, the PSF reports can be downloaded as an overview PDF or CSV files for either the average across all points, or all features individually (D).