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. 2023 Nov 10;12(11):bio059758. doi: 10.1242/bio.059758

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Lateral-X (green), lateral-Y (orange) and axial (yellow) sizes returned by each of the four software packages for each of the six PSFcheck feature sizes imaged. The fluorescent feature sizes increase monotonically from left to right.