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. 2023 Nov 10;12(11):bio059758. doi: 10.1242/bio.059758

Fig. 5.

Fig. 5.

Plots demonstrating the distribution of lateral-X (A), lateral-Y (B) and axial (C) FWHM values determined by three of the four analysis software packages (PyCalibrate – yellow triangles, PSFj – orange circles and MetroloJ QC – green diamonds). The software packages analysed confocal image stacks of six different feature sizes in a PSFcheck slide.