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. 2023 Nov 15;9(46):eadh0322. doi: 10.1126/sciadv.adh0322

Fig. 4. Apc truncation, but not Trp53 loss, aggravates H. pylori–induced DNA damage.

Fig. 4.

(A to F) Antrum-derived organoid cells of the indicated genotypes were seeded in 2D and infected with H. pylori strain G27 (MOI of 50) for 6 hours. Cells were harvested and analyzed for the appearance of DNA damage by alkaline comet assay [(A) and (B)] and by 53bp1/Pcna-specific immunofluorescence microscopy [(C) and (D)]; replication stress was quantified by DNA fiber assay [(E) and (F)]. Representative images of comets, 53bp1 foci formation, and DNA fibers are shown in (A) (scale bars, 100 μm), (C) (scale bar, 10 μm), and (E) (scale bar, 10 μm); pooled data from two to four independent experiments are shown in (B) and (F) [~400 to 600 cells are shown per condition in (B), and 300 fibers are shown per condition in (F)]. A representative experiment of two independently conducted ones is shown in (D) (~1000 cells per condition). Red horizontal lines represent means throughout. P values were calculated using one-way ANOVA; *P < 0.05; **P < 0.01; ****P < 0.001.