Fig. 5. Apc inactivation increases the pool of Pcna⁺ cells in S phase.

(A to C) Antrum-derived organoid cells of the indicated genotypes were seeded in 2D and stained with a Pcna-specific antibody. Representative images are shown in (A) (scale bars, 10 μm), alongside the absolute numbers of Pcna⁺ and Pcna⁻ cells in (B), and the frequency of Pcna⁺ cells among all cells in (C). Three experiments are shown in (B) and (C). Data are plotted as means ± SD. Several thousand cells were analyzed per condition. P values were calculated using one-way ANOVA; *P < 0.05.