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. 2023 Aug 15;21(12):2611–2624. doi: 10.1111/pbi.14157

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© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Functional characterization and catalytic features of Bc7OUGT. (a) Phylogenetic tree of the 46 putative B. chinensis UGTs and other functionally characterized plant UGTs. The tree was adapted from Louveau and Osbourn (2019). (b) Upper panel, UGT phylogenetic subgroups D and E. Lower panel, HPLC analysis of the glycosylation products with tectorigenin using candidate UGTs. (c) Relative expression of Bch3/13400.p1, Bch3/13211.p1, and Bch3/12632.p1 in six organs of B. chinensis. (d) HPLC analysis of the products of bidirectional reactions with tectorigenin or tectoridin using purified Bc7OUGT (denatured Bc7OUGT was used as a negative control). (e) Effects of various temperatures and pH levels on the bidirectional catalytic activity of Bc7OUGT. (f) Subcellular localization of Bc7OUGT in Arabidopsis protoplasts; MGGCFSKK‐mCherry is a membrane localization marker. Scale bars = 10 μm. (g) Conversion rates of Bc7OUGT acting on various flavone substrates.