Primers to screen for the Tg(myl7:dnPI3K) transgene in F1 embryos |
dnPI3K_F1 |
gcgggaagaggacattgact
|
dnPI3K_R1 |
gcgggaagaggacattgact
|
Primers to clone lck-emGFP into the middle-entry vector of the tol2 gateway system |
Hi_lck_1F |
cagtcgactggatccggtacagatccgctagccaccatg
|
Hi_lck_1R |
cagtcgactggatccggtacagatccgctagccaccatg
|
Hi_emgfp_2F |
ggtcgccaccgtgtccaagggcgaggag
|
Hi_emgfp_2R |
ggtcgccaccgtgtccaagggcgaggag
|
Primers to replace actc1b promoter with myl7 promoter in Addgene plasmid 109501 |
Hi_CbPHmkate2_F |
ggctgaaaagcaatcctgcagtgaccaaagcttaaatcagttg
|
Hi_CbPHmkate2_R |
ctctccagaatcactgcggccatggccatggtggctacggatc
|