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. 2023 Nov 3;12:e85930. doi: 10.7554/eLife.85930

Table 1. Primers for genotyping and cloning.

Name Sequence (5′–3′)
Primers to screen for the Tg(myl7:dnPI3K) transgene in F1 embryos dnPI3K_F1 gcgggaagaggacattgact
dnPI3K_R1 gcgggaagaggacattgact
Primers to clone lck-emGFP into the middle-entry vector of the tol2 gateway system Hi_lck_1F cagtcgactggatccggtacagatccgctagccaccatg
Hi_lck_1R cagtcgactggatccggtacagatccgctagccaccatg
Hi_emgfp_2F ggtcgccaccgtgtccaagggcgaggag
Hi_emgfp_2R ggtcgccaccgtgtccaagggcgaggag
Primers to replace actc1b promoter with myl7 promoter in Addgene plasmid 109501 Hi_CbPHmkate2_F ggctgaaaagcaatcctgcagtgaccaaagcttaaatcagttg
Hi_CbPHmkate2_R ctctccagaatcactgcggccatggccatggtggctacggatc