FIG. 1.
Restriction enzyme cleavage of PCR amplification products from 16S rDNA. Cleavage of amplified sequences from isolated strains P. bryantii B14 (lane 2), P. ruminicola 23 (lane 3), P. brevis GA33 (lane 4), P. albensis M384 (lane 5), and B. vulgatus 1447 (lane 6) by HhaI (A), AatII (B), and StuI (C) are shown. Lanes 7 to 13 show HhaI-cut amplification products from rumen samples derived from a cow (lane 7) and from sheep 1 to 4 (lanes 8 to 11) and human and porcine fecal samples (lanes 12 and 13, respectively). Size markers (1-kb ladder [Gibco BRL]) are shown in lanes 1 and 14.