TABLE 1.
Restriction fragment patterns obtained from rumen Prevotella isolates and from colonic Bacteroides spp. after cleavage of an 896- to 898-bp region of 16S rDNA amplified with the rBacPre-plus-fD1 primer set
| Bacterium | Ribotype | Enzyme | Observed band size or database prediction size (bp)a | Database predictionb |
|---|---|---|---|---|
| Bacteroides levii 11028 | 1 | HhaI | 897 | |
| StuI | 897 | |||
| AatII | 897 | |||
| Bacteroides vulgatus 1447 | 2 | HhaI | 794–799, 101–104 | B. forsythus, B. maccacae, B. distasonis,d Porphyromonas salivosa, Porphyromonas oulera |
| Bacteroides vulgatus 10583 | StuI | 892–900 | ||
| Bacteroides uniformis 1004 | AatII | 892–900 | ||
| Bacteroides ovatus 11153 | ||||
| Bacteroides thetaiotaomicron 5482 | 3 | HhaI | 519, 275, 102–104 | B. fragilis |
| StuI | 896–898 | |||
| AatII | 896–898 | |||
| Prevotella ruminicola 23 | 4 | HhaI | 472, 323, 102 | |
| Prevotella ruminicola 118Bc | StuI | 736, 161 | ||
| Prevotella ruminicola TC18c | AatII | 897 | ||
| Prevotella ruminicola TC35c | ||||
| Prevotella ruminicola TC44c | ||||
| Prevotella ruminicola TC27c | ||||
| Prevotella ruminicola TS1-2c | ||||
| Prevotella ruminicola TF1-2c | ||||
| Prevotella bryantii B14 | 5 | HhaI | 472, 266, 102, 57 | P. oralis,d P. disiensd |
| Prevotella bryantii TC1-1c | StuI | 897 | ||
| Prevotella bryantii 92/2c | AatII | 897 | ||
| Prevotella ruminicola TF1-5c | ||||
| Prevotella ruminicola TF1-10c | ||||
| Prevotella brevis GA33 | 6 | HhaI | 472, 323, 102 | |
| Prevotella brevis FC2c | StuI | 897 | ||
| Prevotella brevis FC4c | AatII | 661, 237 | ||
| Prevotella brevis FC6c | ||||
| Prevotella ruminicola-like TC2-24c | ||||
| Prevotella albensis M384c | 7 | HhaI | 529, 158, 108, 102 | P. veroralis, P. melaninogenica |
| Prevotella albensis 52/3c | StuI | 897 | ||
| Prevotella albensis 79/1c | AatII | 897 | ||
| Prevotella ruminicola TC2-3c | 8 | HhaI | 470, 325, 100 | |
| StuI | 900 | |||
| AatII | 900 | |||
| Prevotella brevis-like TS2-7c | 9 | HhaI | 520, 170, 100 | |
| StuI | 900 | |||
| AatII | 900 | |||
| Prevotella ruminicola-like TF2-5c | 10 | HhaI | 470, 325, 100 | |
| StuI | 740 160 | |||
| AatII | 660 240 | |||
| Prevotella ruminicola-like 223/M2/7c | 11 | HhaI | 510, 160, 110, 100, 20 | |
| StuI | 900 | |||
| AatI | 900 |
The rBacPre terminal fragment is indicated in boldface type.
Additional species predicted to give the same ribotype with respect to the rBacPre terminal fragment; other species predicted to belong to additional ribotypes (not shown) include B. eggerthii, B. heparinolytica, B. zoogleoformans, P. oris, P. buccae, P. bivia, P. buccalis, P. loeschii, P. intermedia, P. denticola, P. corporis, P. nigrescens, and Porphyromonas gingivalis.
Full 16S rDNA sequence not available; either approximate fragment sizes or the fragment sizes for sequenced representatives of the same ribotype are shown.
Predicted to show the same rBacPre terminal fragment, but differing in other HhaI digest products.