TABLE 3.
Changes in Bacteroides and Prevotella ribotypes with diet and sampling time in rumen liquor from two sheep
| Sample and source | Sampling timea | Dietb | % of Prevotella and Bacteroides 16S rDNA present as ribotype:
|
% of Prevotella and Bacteroides in total eubacterial 16S rDNA | |||||
|---|---|---|---|---|---|---|---|---|---|
| 1 + 2 | 4 | 5 | 6 | 7 + 11 | 8 | ||||
| Rumen, sheep 5 | Pre | 3 | 12.7 | 15.0 | 8.4 | 5.4 | 14.7 | 43.7 | 62.0 |
| Post | 3 | 14.1 | 11.4 | 9.0 | 3.3 | 15.4 | 46.7 | 45.0 | |
| Pre | 4 | 42.2 | 7.3 | 21.6 | 0.0 | 0.0 | 28.7 | 54.0 | |
| Post | 4 | 42.7 | 14.8 | 21.5 | 0.0 | 10.2 | 10.8 | 42.0 | |
| Rumen, sheep 6 | Pre | 3 | 10.8 | 4.5 | 49.5 | 29.3 | 6.5 | 0.0 | 45.0 |
| Post | 3 | 12.3 | 3.0 | 55.4 | 26.2 | 1.6 | 1.5 | 49.0 | |
| Pre | 4 | 30.3 | 7.9 | 12.2 | 1.7 | 22.8 | 20.1 | 57.0 | |
| Post | 4 | 44.1 | 3.2 | 7.7 | 2.6 | 21.3 | 21.4 | 60.0 | |
a
Pre, samples taken immediately before feeding; Post, samples taken 2 h after feeding.
b
The two animals were first fed diet 3 and then switched to diet 4 (see the text) and sampled again 28 days later.