Figure 1.

Survival of macaques and comparison of viral loads after BDBV challenge and postexposure treatment with rVSVΔG/BDBV-GP. A, Study design and survival curve of BDBV-exposed cynomolgus macaques left untreated (n = 1) or treated with rVSVΔG/BDBV-GP (n = 6). Historical untreated controls are also depicted (n = 13). All animals were infected intramuscularly with a target dose of 1000 PFU of BDBV-Uganda and then 6 animals were treated with rVSVΔG/BDBV-GP 20-23 minutes later. B, Clinical scores of BDBV-infected macaques; criteria include behavior, posture and activity level, appetite, respiration, and the presence of hemorrhagic manifestations. C, Circulating viral RNA. Measured by RT-qPCR in whole blood and reported as log₁₀ copies/mL. The limit of detection for this assay was 1000 copies/mL. D, Viral loads were measured in plasma samples by standard plaque assay and reported as log₁₀ PFU/mL. The limit of detection for this assay was 25 PFU/mL. Individual treated subjects are denoted by the following symbols: fatal treated, diamond; survivor 3, triangle; survivor 4, square. Abbreviations: BDBV, Bundibugyo virus; dpi, days postinfection; GP, glycoprotein; PFU, plaque-forming unit; RT-qPCR, quantitative real-time polymerase chain reaction; rVSV, recombinant vesicular stomatitis virus.