Figure 2.

Antibody responses to HPIV3/ΔF-HN-EboGP, HPIV3/ΔF-HN/SUDV-GP, and HPIV3/ΔF-HN/Trivalent in guinea pigs. A, Binding IgG evaluated by ELISA. B, Binding IgA evaluated by ELISA. C, Virus-neutralizing antibody titers determined by plaque reduction assay. The GP antigens for ELISA or viruses for neutralization are indicated at the top of the figure, and the vaccines or vector control are indicated at the bottom. The dotted line indicates the level of nonspecific binding in pooled samples collected before vaccination. n = 5 except the HPIV3 group in EBOV and BDBV studies where n = 4. P values were determined by Kruskal-Wallis test followed by Dunn postcomparisons test. Abbreviations: BDBV, Bundibugyo virus; EBOV, Ebola virus; ELISA, enzyme-linked immunosorbent assay; GP, glycoprotein; HPIV3, human parainfluenza virus type 3; Ig, immunoglobulin; SUDV, Sudan virus.