Figure 3.

Antibody responses to HPIV3/ΔF-HN/BDBV-GP and HPIV3/ΔF-HN/Trivalent in ferrets. A, Binding IgG evaluated by ELISA. B, Binding IgA evaluated by ELISA. C, Virus-neutralizing antibody titers determined by plaque reduction assay. The GP antigens for ELISA or viruses for neutralization are indicated at the top of the figure, and the vaccines or vector control are indicated at the bottom. Pooled serum samples collected prior to vaccination demonstrated no detectable binding. n = 5 animals per group except for IgA assays where n = 2 for HPIV3. P values were determined by Kruskal-Wallis test followed by Dunn postcomparisons test. Abbreviations: BDBV, Bundibugyo virus; EBOV, Ebola virus; ELISA, enzyme-linked immunosorbent assay; GP, glycoprotein; HPIV3, human parainfluenza virus type 3; Ig, immunoglobulin; SUDV, Sudan virus.