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. 2005 Mar;71(3):1522–1530. doi: 10.1128/AEM.71.3.1522-1530.2005

TABLE 1.

Purification of rNROR

Fraction Protein (mg) Sp. act. (U/mg)a Total U
Cell extract 2,597 8.1 21,040
Chitin resin 15.2 8,460 128,600
Concentrated 11.2 12,913 144,600
a

Units are defined as micromoles of Rd reduced per minute by rNROR. Assays were performed at 80°C in 100 mM EPPS buffer (pH 8.0) using recombinant P. furiosus Rd.