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. 2005 Mar;71(3):1522–1530. doi: 10.1128/AEM.71.3.1522-1530.2005

TABLE 2.

Reconstitution of recombinant P. furiosus NROR

Sample Sp. act. (U/mg)
As purified apoenzymea 3.5
Heat treated, without FAD 21.9
Heat treated, with FAD 352.0
Heat treated, with FMN <0.1
a

For the reconstitution experiments, rNROR was purified from the chitin-agarose bead column without the addition of 0.1 mM FAD in the cleavage buffer, as described in Materials and Methods.