TABLE 4.

In vitro reconstitution of the P. furiosus NAD(P)H-dependent superoxide reduction pathway as monitored by hydrogen peroxide production

Additions to reaction mixturea	Amt of hydrogen peroxide (μmol)
SOD	0.36
Dithionite-reduced SOR	0.48
SOR, NROR, Rd, NADPH	0.81
SOR, NROR, NADPH	0.09
SOR, Rd, NADPH	0.16
NROR, Rd, NADPH	0.09

^aAll assays were performed at 23°C. The reaction mixture contained a superoxide-generating system (xanthine oxidase, xanthine, and horse heart cytochrome c). See Materials and Methods for details. Where indicated, bovine SOD (30 U), NADPH (0.3 mM), SOR, NROR, and/or Rd from P. furiosus (each 170 nM) were added.