TABLE 1.
Summary of real-time-t-RFLP analysis of a three-member bacterial community
| No. of DNA templates per PCR mixture, ng/PCR reaction (82A/103A/105A ratio)a | No. of 16S rRNA gene copies/mlb
|
||
|---|---|---|---|
| E. coli | B. thuringiensis | P. fluorescens | |
| 0.5 (1.1:1.1:1) | (0.96 ± 0.04) × 106 | (1.18 ± 0.05) × 106 | (0.79 ± 0.15) × 106 |
| 5 (1.0:1.1:1) | (1.28 ± 0.15) × 107 | (1.32 ± 0.52) × 107 | (0.82 ± 0.06) × 107 |
| 10 (0.9:0.9:1) | (2.87 ± 0.46) × 107 | (2.76 ± 0.42) × 107 | (2.26 ± 0.45) × 107 |
| 25 (0.9:0.8:1) | (7.41 ± 1) × 107 | (6.59 ± 0.18) × 107 | (6.00 ± 0.08) × 107 |
a
82A, area under 82-bp T-RF, representing E. coli; 103A, area under 103-bp T-RF, representing B. thuringiensis; 105A, area under 105-bp T-RF, representing P. fluorescens.
b
The values were calculated by performing data analysis as described in Materials and Methods and indicate the ranges for duplicate experiments.