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. 2005 Mar;71(3):1553–1561. doi: 10.1128/AEM.71.3.1553-1561.2005

FIG. 3.

FIG. 3.

In situ identification of bacterial symbionts in O. crassitunicatus. The epifluorescence images show cross sections through the entire worm (a) (scale bar, 20 μm) and the symbiont-containing region of the worm's body wall (b to f) (scale bars, 10 μm). All worms shown were from station A; the only exception is the worm shown in panel c, which was from station B. (a) Dual hybridization with the GAM42a and DSS658/DSR651 probes, showing γ-proteobacterial symbionts (red) and δ-proteobacterial symbionts (green). (b) Dual hybridization with the OcraGAM1 and OcraGAM2 probes, showing the Gamma 1 symbionts (red) and Gamma 2 symbionts (green). The inset shows an enlargement of the area enclosed by a box (scale bar, 5 μm). (c) Dual hybridization with the OcraDEL1 and OcraDEL2 probes, showing the Delta 1 symbionts (green) and Delta 2 symbionts (red). (d) Dual hybridization with the OcraDEL1/OcraDEL2 and OcraDEL3 probes, showing the Delta 1 and Delta 2 symbionts (red)and the Delta 3 symbionts (green). The inset shows an enlargement of the area enclosed by a box area (scale bar, 5 μm). (e) Monohybridization with the OcraSPI probe, showing spirochete symbionts (green). (f) Triple hybridization with the GAM42a, DSS658/DSR651, and OcraSPI probes, showing the two γ-proteobacterial symbionts (red), the three δ-proteobacterial symbionts (blue), and the spirochete symbionts (yellow). The muscle tissue of the worm at the bottom of the panel appears to be bluish green because of autofluorescence at mixed wavelengths.