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. 2005 Mar;71(3):1223–1230. doi: 10.1128/AEM.71.3.1223-1230.2005

TABLE 1.

Strains and plasmids used in this work

Strain or plasmid Relevant characteristic(s)a Reference
Strains
    Escherichia coli DH5-α Plasmid-free strain 25
    Lactobacillus plantarum WCFS1 Single-colony isolate of NCIMB8826, sequenced strain 35
    Lactobacillus plantarum WCFS2 Strain WCFS1 with plasmid pWCFS110 instead of pWCFS103 This study
    Lactobacillus plantarum WCFS3 Strain WCFS1 with plasmid pWCFS111 instead of pWCFS103 This study
    Lactobacillus plantarum WCFS4 Strain WCFS1 with plasmid pWCFS112 instead of pWCFS103 This study
    Lactobacillus plantarum NC8 Plasmid-free strain 5
    Lactobacillus plantarum NC8R Rif derivative of NC8 This study
    Lactobacillus plantarum NZ7109 Eryr derivative of WCFS1 9
    Lactobacillus casei LMG6904 Lactobacillus casei type strain (ATCC 393) 26
    Lactobacillus helveticus CNRZ 32 Plasmid-free strain 33
    Lactococcus lactis MG1363 Plasmid-free strain 22
    Leuconostoc lactis NZ6091 Plasmid-free strain 13
    Streptococcus thermophilus ST11 Plasmid-free strain 42
    Carnobacterium maltaromaticum LV17B Plasmid-free strain 1
    Bacillus subtilis 168 Plasmid-free strain 53
Plasmids
    pUC18Ery Ampr Eryr, 3.6-kb pUC18 integration vector carrying the erythromycin resistance gene from pAMβ1 57
    pNZ124 Cmr, 2.8-kb pSH71 replicon 46
    pWCFS101 1,917-bp cryptic plasmid This study
    pWCFS102 2,365-bp cryptic plasmid This study
    pWCFS103 36,069-bp conjugative arsenate or arsenite resistance plasmid This study
    pWCFS104 Cmr, 3.0-kb, pWCFS101-based cloning vector This study
    pWCFS105 Cmr, 3.4-kb, pWCFS102-based cloning vector This study
    pWCFS106 Cmr, 4.2-kb, pWCFS103-based cloning vector This study
    pWCFS107 Eryr, arsB integration vector This study
    pWCFS108 Eryr, cadD integration vector This study
    pWCFS109 Eryr, traA integration vector This study
    pWCFS110 Eryr, arsB, pWCFS107 integrated into the arsB gene of pWCFS103 This study
    pWCFS111 Eryr, cadD, pWCFS108 integrated into the cadD gene of pWCFS103 This study
    pWCFS112 Eryr, traA, pWCFS109 integrated into the traA gene of pWCFS103 This study
a

Ampr, ampicillin resistant; Eryr, erythromycin resistant; Tetr, tetracycline resistant, Cmr, chloramphenicol resistant; Rifr, rifampin.