TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s)a | Reference | ||
|---|---|---|---|---|
| Strains | ||||
| Escherichia coli DH5-α | Plasmid-free strain | 25 | ||
| Lactobacillus plantarum WCFS1 | Single-colony isolate of NCIMB8826, sequenced strain | 35 | ||
| Lactobacillus plantarum WCFS2 | Strain WCFS1 with plasmid pWCFS110 instead of pWCFS103 | This study | ||
| Lactobacillus plantarum WCFS3 | Strain WCFS1 with plasmid pWCFS111 instead of pWCFS103 | This study | ||
| Lactobacillus plantarum WCFS4 | Strain WCFS1 with plasmid pWCFS112 instead of pWCFS103 | This study | ||
| Lactobacillus plantarum NC8 | Plasmid-free strain | 5 | ||
| Lactobacillus plantarum NC8R | Rif derivative of NC8 | This study | ||
| Lactobacillus plantarum NZ7109 | Eryr derivative of WCFS1 | 9 | ||
| Lactobacillus casei LMG6904 | Lactobacillus casei type strain (ATCC 393) | 26 | ||
| Lactobacillus helveticus CNRZ 32 | Plasmid-free strain | 33 | ||
| Lactococcus lactis MG1363 | Plasmid-free strain | 22 | ||
| Leuconostoc lactis NZ6091 | Plasmid-free strain | 13 | ||
| Streptococcus thermophilus ST11 | Plasmid-free strain | 42 | ||
| Carnobacterium maltaromaticum LV17B | Plasmid-free strain | 1 | ||
| Bacillus subtilis 168 | Plasmid-free strain | 53 | ||
| Plasmids | ||||
| pUC18Ery | Ampr Eryr, 3.6-kb pUC18 integration vector carrying the erythromycin resistance gene from pAMβ1 | 57 | ||
| pNZ124 | Cmr, 2.8-kb pSH71 replicon | 46 | ||
| pWCFS101 | 1,917-bp cryptic plasmid | This study | ||
| pWCFS102 | 2,365-bp cryptic plasmid | This study | ||
| pWCFS103 | 36,069-bp conjugative arsenate or arsenite resistance plasmid | This study | ||
| pWCFS104 | Cmr, 3.0-kb, pWCFS101-based cloning vector | This study | ||
| pWCFS105 | Cmr, 3.4-kb, pWCFS102-based cloning vector | This study | ||
| pWCFS106 | Cmr, 4.2-kb, pWCFS103-based cloning vector | This study | ||
| pWCFS107 | Eryr, arsB integration vector | This study | ||
| pWCFS108 | Eryr, cadD integration vector | This study | ||
| pWCFS109 | Eryr, traA integration vector | This study | ||
| pWCFS110 | Eryr, arsB, pWCFS107 integrated into the arsB gene of pWCFS103 | This study | ||
| pWCFS111 | Eryr, cadD, pWCFS108 integrated into the cadD gene of pWCFS103 | This study | ||
| pWCFS112 | Eryr, traA, pWCFS109 integrated into the traA gene of pWCFS103 | This study |
a
Ampr, ampicillin resistant; Eryr, erythromycin resistant; Tetr, tetracycline resistant, Cmr, chloramphenicol resistant; Rifr, rifampin.