TABLE 2.
Albicidin binding activity of AlbA and its variants
| Strain (single-site mutation) | AlbA | K106A | W110A | Y113A | L114A | H125A | Y126A | P134A | W162A |
|---|---|---|---|---|---|---|---|---|---|
| Relative activity (%)a | 100 ± 0.0 | 72.1 ± 2.5 | 77.8 ± 4.6 | 72.6 ± 5.6 | 73.9 ± 4.4 | 68.6 ± 5.8 | 41.7 ± 5.7 | 70.2 ± 2.3 | 73.9 ± 4.4 |
| Strain (double-site mutations) | AlbA | K106A, H125A | W110A, H125A | Y113A, H125A | L114A, H125A | H125A, Y126A | H125A, P134A | H125A, W162A |
| Relative activity (%)a | 100 ± 0.0 | 37.1 ± 3.1 | 48.5 ± 2.6 | 52.9 ± 4.2 | 56.7 ± 3.2 | 21.4 ± 4.6 | 62.9 ± 2.8 | 67.4 ± 3.5 |
a
Relative albicidin binding activities were determined by the bioassay described in Materials and Methods. Data shown are means ± standard deviations.