TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| LLB1 | supE Δthi (lac-proAB) hsdD5 (F′ traD36 proAB lacIqZΔM15) pcnB | 14 |
| TG1 RepA | TG1 with repA from E. coli EC101 integrated in the chromosome | 9 |
| L. casei | ||
| DN-114 001 | L. casei Danone strain | CNCMa no. I-1518 |
| DN-240 041 | DN-114 001, Emr, lacTp*::luxAB on pDN26 integrated in the chromosome (*lacT promoter without the ribonucleic antiterminator sequences) (1) | 18 |
| DN-240 047 | DN-114 001, Emr, ccpAp::luxAB on pDN75 integrated in the chromosome | This study |
| DN-240 048 | DN-114 001, Emr, dltp::luxAB on pDN76 integrated in the chromosome | This study |
| DN-240 049 | DN-114 001, Emr, ldhp::luxAB on pDN77 integrated in the chromosome | This study |
| Plasmids | ||
| pBluescript II | Ampr, M13ori pBR322ori | Stratagene |
| pDN69 | Ampr, 241-bp fragment of L. casei containing ccpA cloned in pBluescript II | This study |
| pDN70 | Ampr, 267-bp fragment of L. casei containing dlt cloned in pBluescript II | This study |
| pDN71 | Ampr, 525-bp fragment of L. casei containing ldh cloned in pBluescript II | This study |
| pIL4232 | Ampr, luxAB genes from P. luminescens cloned in pBluescript II | This study |
| pIL4240 | Ampr Emr, fusion of pIL4232 with pWV01(ΔrepA) | This study |
| pIL4242 | Emr, ori (pWV01 ΔrepA), integrative vector derived from pIL4240 | This study |
| pDN72 | Ampr Emr, fusion of pDN69 and pIL4242 | This study |
| pDN73 | Ampr Emr, fusion of pDN70 and pIL4242 | This study |
| pDN74 | Ampr Emr, fusion of pDN71 and pIL4242 | This study |
| pDN75 | Emr, derivative of pDN72 carrying ccpAp::luxAB | This study |
| pDN76 | Emr, derivative of pDN73 carrying dltp::luxAB | This study |
| pDN77 | Emr, derivative of pDN74 carrying ldhp::luxAB | This study |
a
CNCM, Collection Nationale de Culture de Microorganisms.