TABLE 2.
Primers used in this study
| Primer | Accession no. | Sequence | Descriptiona |
|---|---|---|---|
| Promoter amplification | |||
| OLC1 | U28137 | 5′-GCTCTAGAGCTCGCGATCTGGCTTAAAAG-3′ | ccpAp Fwd |
| OLC2 | U28137 | 5′-TCCCCCCGGGCCAATTGATTTTCTCCTTAGTCG-3′ | ccpAp Rev |
| OLC3 | M83993 | 5′-GCTCTAGACGTGGGGTGACAAGATGTTC-3′ | dltp Fwd |
| OLC8 | M83993 | 5′-TCCCCCCGGGGATCAATCGCTGTAATAACATTATC-3′ | dltp Rev |
| OLC39 | AF351136 | 5′-CCGGAATTCTGTGCGATTGCTTTGAGATT-3′ | ldhp Fwd |
| OLC41 | AF351136 | 5′-CGCGGATCCGAATAACTTTTTGGTGATCC-3′ | ldhp Rev |
| Integrant analysis | |||
| OLC33 | M90093 | 5′-GATATGAATATGCTGAAAGATA-3′ | luxB Fwd |
| OLC52 | U28137 | 5′-GCTAAACCAGCTTGGTAGCT-3′ | ccpA Rev |
| OLC71 | M83993, AAAZ01000003, AP004824 | 5′-CCATCACGNATTTCCATAAT-3′ | dlt Rev |
| OLC72 | AF351136, AAK01000273, U78634, AF054624, AF436734, M72545 | 5′-TCMARTGAAGTACCTGARCC-3′ | ldh Rev |
| Luciferase and other primers | |||
| OLC15 | M90093 | 5′-CGGGATCCAAATTTGGAAACTTTTTGCTTACA-3′ | luxA Fwd |
| OLC16 | M90093 | 5′-AATCTGCAGCTAATATAATAGCGAACGTTGTT-3′ | luxA Rev |
| OLC17 | M90093 | 5′-CGGGATCCAAATTTGGATTGTTCTTCCTTAA-3′ | luxB Fwd |
| OLC18 | M90093 | 5′-AATCTG CAGTTAGGTATATTCCATGTGGTAC-3′ | luxB Rev |
| P1 | See kit | 5′-GCTTCCGGCTCGTATGTTGTGTGG-3′ | T7p |
Fwd, forward; Rev, reverse.