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EMSA of AflR1 sites in nor-1 and ver-1 promoters. Twenty femtomoles of radiolabeled oligonucleotides carrying AflR1 from the nor-1 and ver-1 promoters was subjected to EMSA in the presence of an AflR::MBP (AM) fusion protein (0.4 μg per reaction mixture), MBP (M) (as a control; 16 μg per reaction mixture), or no protein (N) (see Materials and Methods). The arrow indicates the location of the specific AflR::MBP/ver-1 oligonucleotide complex.
