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. 2005 Mar;71(3):1267–1275. doi: 10.1128/AEM.71.3.1267-1275.2005

FIG. 1.

FIG. 1.

Demonstration of RNA purification, poly(A) tail modification, and RT-PCR amplification of environmental RNA. (A) Denaturing agarose (1%) gel containing electrophoresed samples resulting from RNA extraction, purification, and modification with poly(A)polymerase. Lane 1, molecular weight markers; lane 2, RNA extracted from soil and purified; lane 3, DNase-treated and gel-purified RNA; lane 4, soil RNA after poly(A) tailing reaction. The bracket indicates the position of the various tailed RNA molecules. The locations of 23S and 16S RNAs are indicated by asterisks and were determined by examining numerous reproducible gels in which these bands were aligned with the corresponding bands of RNA purified from E. coli (data not shown) and by molecular weight, as shown. (B) Final PCR product electrophoresed in a 1% agarose gel. Lane 1, molecular weight markers; lane 2, cDNA product from PCR amplification. The arrow indicates the position of the PCR product. In both panels, the sizes of all molecular weight markers are indicated on the left.