Table 8.
Fab concentrations determined by an antigen quantitative ELISA. Overall yields and recovery were calculated as a result of two-step purification from two different batches.
Two-step Purification | Fraction | Fab Amount (mg) | Recovery (%) | Total Process Recovery (%) |
---|---|---|---|---|
Purification | Load | 1077.2 ± 58.8 | 71.485 ± 3.475 | 51.2 ± 0.56 |
Final | 772.189 ± 79.5 | |||
TFF | Load | 770.149 ± 79.4 | 71.89 ± 2.67 | |
Final | 551.89 ± 36.2 |