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. 2023 Apr 6;7(11):1392–1403. doi: 10.1038/s41551-023-01015-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Illustrative scatter plot of brightness average versus cell size for cells isolated from the liver showing numerous clusters of cells. The marked population of cells (forming a cluster of cell size 25–50 μm² and brightness average 100–115) is enriched for EpCAM-positive (epithelial) cells but devoid of CD31-positive (endothelial) cells or CD45-positive cells (leukocytes). FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin. b, Illustrative scatter plots of colon cells stained for EpCAM and CD45 cell surface markers. Within the EpCAM-positive population, seven subpopulations of cells can be identified on the basis of density plots of physical parameters, such as brightness and cell size. c, Illustrative scatter plots of kidney cells stained for EpCAM and CD45. Within the CD45 population, four subpopulations are identified based on similarities in cell size and deformation. The colour map in the scatter plots represents the event density.