Extended Data Fig. 1. Comparison of cell viability and cell yield of mechanical vs standard dissociation of different murine tissues.
a,b, Percentage of viable cells for different organs dissociated using a tissue grinder (TG; marked in red) or standard dissociation (marked in blue). Cell viability was assessed using (a) propidium iodide and RT-FDC and (b) trypan blue exclusion assay. c, Number of cells (obtained using a cell counter device) per mg of tissue processed. Lung, liver, kidney, pancreas and stomach processed with enzymatic dissociation were not weighted prior to the experiments. The line represents the mean and the box extends from minimum to maximum values. TG data kidney and spleen: n = 5, all other organs n = 5; for enzymatic dissociation kidney, pancreas, stomach: n = 3; all other organs n = 4; biologically independent repeats performed as independent experiments).