Extended Data Fig. 2. PHM in the rostral–caudal direction, but not left–right, direction lowers blood pressure in SHRSPs.
a, Schematic representation of the experimental protocol to analyse the effects of PHM on the blood pressure in rats. The cartoon represents PHM in the dorsal-ventral (left), rostral-caudal (centre), and left-right (right) directions. b,c, Time courses (b) and values on Day 29 (c) of MAP in SHRSPs, subjected to either daily PHM or anaesthesia only [b, orange vs. blue: P = 0.0376 for Day 15, P = 0.0018 for Day 22, P = 0.0105 for Day 29; orange vs. magenta: P = 0.4131 for Day 15, P = 0.2448 for Day 22, P = 0.0148 for Day 29; orange vs. green: P = 0.4869 for Day 15, P = 0.6439 for Day 22, P = 0.9273 for Day 29. c: P = 0.0363 for column 1 vs. 2, P = 0.0465 for column 1 vs. 3, P = 0.9576 for column 1 vs. 4, P = 0.9948 for column 2 vs. 3. n = 8 rats for PHM (−) and PHM (dorsal-ventral); n = 5 rats for PHM (rostral-caudal) and PHM (left-right)]. The data for columns 1 and 2 in (c) are respectively identical to those for columns 3 and 4 in Fig. 1c. Data are presented as mean ± s.e.m. *P < 0.05; **P < 0.01; NS, not significant; two-way repeated measures ANOVA with Dunnett’s post hoc multiple comparisons test (b) or one-way ANOVA with Tukey’s post hoc multiple comparisons test (c).