Extended Data Fig. 4. Treadmill running decreases AT1R expression in the RVLM astrocytes in SHRSPs.
a, Micrographic images of anti-NeuN for mature neurons (blue), anti-GFAP for glial fibrillary acidic protein-positive astrocytes (green) and anti-AT1R for angiotensin II type 1 receptor (red) immunostaining of the RVLM of SHRSPs, either placed in the static treadmill machine or subjected to treadmill running at a velocity of 20 m/min (30 min/day, 28 days). Arrows point to anti-AT1R immunosignals that overlap with anti-GFAP, but not anti-NeuN, immunosignals in merged images. Scale bar, 50 μm. Three images for treadmill (−) are from a particular section of a rat whereas those for treadmill (+) are from a different individual rat. They are representative of three [treadmill (−)] or four [treadmill (+)] rats. b,c, Quantification of AT1R-positive neurons (b) and astrocytes (c) in the RVLM of SHRSPs with or without 4-week treadmill running. Fifty NeuN+ cells and 100 GFAP+ cells were analysed for each rat [b: P = 0.7056. c: P = 0.0048. n = 3 rats for treadmill (−); n = 4 rats for treadmill (+)]. Data are presented as mean ± s.e.m. **P < 0.01; NS, not significant, unpaired two-tailed Student’s t-test.