Extended Data Fig. 1. Daily anaesthesia alone does not affect blood pressure, and ≥ 4 weeks of daily PHM decreases or delays stroke incidence in SHRSPs.
a, Time courses of MAP in SHRSPs either routinely kept in cages (no anaesthesia) or subjected to daily anaesthesia on the platform of PHM machine without turning on its switch [PHM (−)] [P > 0.9999 for Day 15, Day 22, and Day 29. n = 6 rats for no daily anaesthesia; n = 8 rats for PHM (−)]. b, Schematic representation of the experimental protocol to analyse the effects of PHM on the stroke incidence in SHRSPs. c,d, Kaplan-Meier curve of the stroke incidence in SHRSPs with and without PHM represented as PHM (+) and PHM (−), respectively. Data from SHRSPs that did not have a stroke during the first four weeks after the initiation of intervention (daily PHM or anaesthesia alone) is shown in (d) [c: P = 0.1179. n = 15 rats for PHM (−); n = 15 rats for PHM (+). d: P = 0.0093. n = 9 rats for PHM (−); n = 8 rats for PHM (+)]. Data are presented as mean ± s.e.m. **P < 0.01; NS, not significant; two-way repeated measures ANOVA with Bonferroni’s post hoc multiple comparisons test (a) or long-rank test (c,d).