(A)
DUSP4 mRNA expression in DOCK1-knockout HTR-8 cells. (B) DUSP4 protein levels in DOCK1-depleted HTR-8 cells (left). The protein levels were quantified (right). (C) Wound healing ability after DUSP4 inhibition by siRNA in DOCK1-knockout HTR-8 cells (left). Rate of wound closure was quantified (right). Scale bar: 200 μm. (D) Migration and invasion ability after DUSP4 inhibition in DOCK1 knockout HTR-8 cells (top). The number of migrating and invading cells was quantified (bottom). Scale bar: 100 μm. (E) ERK phosphorylation and metastasis-related protein levels after DUSP4 siRNA treatment in DOCK1-knockout HTR-8 cells. In (A, C, D), each group, n = 3 biological replicates (*P < 0.05; **P < 0.01; unpaired two-tailed t test). In (B), each group, n = 4 biological replicates (***P < 0.001; unpaired two-tailed t test).