TABLE 2.
Thirty primer combinations screened during initial testing to determine optimal primer sets for AFLP typing of C. botulinum
| Primer specific to rare-cutting restriction site sequencea,b | Primer specific to frequent-cutting restriction site sequencea |
|---|---|
| Eco-C | Hpy-C |
| Eco-C | Hpy-G |
| Apa-0 | Hpy-A |
| Apa-0 | Hpy-C |
| Apa-0 | Hpy-G |
| Apa-A | Hpy-A |
| Apa-C | Hpy-0 |
| Apa-C | Hpy-A |
| Apa-G | Hpy-0 |
| Apa-G | Hpy-A |
| Hind-0 | Mse-A |
| Hind-0 | Mse-C |
| Hind-0 | Mse-G |
| Hind-A | Mse-A |
| Hind-A | Mse-G |
| Hind-C | Mse-A |
| Hind-C | Mse-C |
| Hind-C | Mse-G |
| Hind-0 | Hpy-A |
| Hind-0 | Hpy-C |
| Hind-0 | Hpy-G |
| Hind-A | Hpy-A |
| Hind-A | Hpy-C |
| Hind-A | Hpy-G |
| Hind-C | Hpy-A |
| Hind-C | Hpy-C |
| Hind-C | Hpy-G |
| Hind-G | Hpy-0 |
| Hind-G | Hpy-A |
| Hind-G | Hpy-C |
a
0, no selective extension; A, C, or G, selective nucleotide at the 3′ end of the primer.
b
IRD800-labeled primer.