TABLE 3.
Adapter and primer oligonucleotides used in AFLP
| Oligonucleotide | Sequence(s) |
|---|---|
| AFLP adapters | |
| Apa adapter | 5′-TCGTAGACTGCGTACAGGCC-3′ and 3′-CATCTGACGCATGT-5′ |
| Eco adapter | 5′-CTCGTAGACTGCGTACC-3′ and 3′-CTGACGCATGGTTAA-5′ |
| Hind adapter | 5′-CTCGTAGACTGCGTACC-3′ and 3′-CTGACGCATGGTCGA-5′ |
| Hpy adapter | 5′-GACGATGAGTCCTGAC-3′ and 3′-TACTCAGGACTGGC-5′ |
| Mse adapter | 5′-GACGATGAGTCCTGAG-3′ and 3′-CTACTCAGGACTCAT-5′ |
| Core sequences of AFLP primersa | |
| Apa primer | 5′-GACTGCGTACAGGCCC-3′ |
| Eco primer | 5′-GACTGCGTACCAATTC-3′ |
| Hind primer | 5′-GACTGCGTACCAGCTT-3′ |
| Hpy primer | 5′-CGATGAGTCCTGACCGT-3′ |
| Mse primer | 5′-GATGAGTCCTGAGTAA-3′ |
a
No selective bases or an adjacent A, C, or G selective nucleotide at the 3′ end of the primer.