TABLE 1.
Oligonucleotides used in this study
| Amplicon | 5′ Oligonucleotide | 3′ Oligonucleotide | Amplicon size (bp) | Primer pair efficiency (%) |
|---|---|---|---|---|
| M. medusae ITS | TGAGTTGCTAAATGCATTC | TAAGCCAAAGTTGCCTTTGTC | 119 | 97.4 |
| M. larici-populina ITS | TGAGCGACTTTAATGTGACTC | ATGTAAATCAAAGTTGCCTTTGCG | 123 | 91.3 |
| Poplar | GAAGTAGCACTTGCTGCATAG | GTCAAAATCTTGGGAGTCAAAG | 122 | 100 |
| Tubulina | TGGGAAGTGGTGTCTGATGA | GATCGAACGGAATCCATTGT | 370/381b | NDc |
| Nested tubulin | ||||
| M. medusae | ACACTGATTCGTTCGAGTTG | 186 | 84.4 | |
| M. larici-populina | ACGCTGATTCGCTCGAGTTG | 184 | 84.8 |
a
The same primer pair was used for both M. medusae and M. larici-populina.
b
M. medusae amplicon size/M. larici-populina amplicon size.
c
ND, not determined.