TABLE 3.
M. medusae primer pair specificity evaluated by comparing Ct values in mixed DNA samples
| Amt of M. medusae DNAa (ng) | Amt of M. larici-populina DNAa (ng)
|
Avgb | |||
|---|---|---|---|---|---|
| 0 | 0.05 | 0.5 | 5 | ||
| 0 | Nonec | None | None | None | None |
| 0.05 | 20.7 ± 0.3 | 20.7 ± 0.4 | 20.8 ± 0.1 | 20.9 ± 0.1 | 20.8 ± 0.2 |
| 0.5 | 17.4 ± 0.3 | 17.5 ± 0.4 | 17.5 ± 0.2 | 17.7 ± 0.3 | 17.5 ± 0.3 |
| 5 | 14.0 ± 0.2 | 13.9 ± 0.1 | 14.0 ± 0.5 | 13.9 ± 0.3 | 14.0 ± 0.3 |
a
DNA was extracted from infected clone Jackii leaves at 10 days postinfection. The mixtures contained 0, 0.05, 0.5, or 5 ng of DNA isolated from M. medusae- or M. larici-populina-infected leaves. Amplification was carried out in triplicate with the M. medusae ITS primer pair.
b
Average for all 12 runs.
c
None, no amplification detected.