TABLE 4.

M. larici-populina primer pair specificity evauated by comparing C_t values in mixed DNA samples

Amt of M. medusae DNAa (ng)	Amt of M. larici-populina DNAa (ng)
	0	0.05	0.5	5
0	Noneb	21.4 ± 0.3	18.2 ± 0.8	14.3 ± 0.4
0.05	None	21.7 ± 0.4	18.1 ± 0.5	14.5 ± 0.7
0.5	None	21.2 ± 0.3	18.0 ± 0.4	14.4 ± 0.4
5	None	21.5 ± 0.4	18.1 ± 0.4	14.5 ± 0.5
Avgc	None	21.4 ± 0.4	18.1 ± 0.5	14.4 ± 0.4

^aDNA was extracted from infected clone Jackii leaves at 10 days postinfection. The mixtures contained 0, 0.05, 0.5, or 5 ng of DNA isolated from M. medusae- or M. larici-populina-infected leaves. Amplification was carried out in triplicate with the M. larici-populina ITS primer pair.

^bNone, no amplification detected.

^cAverage for all 12 runs.