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. 2005 Mar;71(3):1642–1647. doi: 10.1128/AEM.71.3.1642-1647.2005

FIG. 1.

FIG. 1.

Purification of heterologously expressed XR-His6-tagged fusion protein. Lane 1, the molecular weight marker; lane 2, the soluble fraction of the induced cells; lane 3, the flowthrough during purification; lane 4, final purified XR. The soluble expression of the XR reaches very high levels of around 50% of the total cellular protein. MW, molecular weight.