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(A) kcat dependence on temperature. XR was assayed at different temperatures from 13 to 65°C. (B) Thermal inactivation of XR at 40°C. The heat inactivation at 40°C was irreversible and followed first-order kinetics with a half-life of 94 min. (C) pH rate profile. Saturating concentrations of 1 M xylose and 200 μM NADPH were used to measure the activity in a universal buffer at various pH values from 3.5 to 8.0.
