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Parameters for N. crassa XRa
| N. crassa XR with indicated coenzyme | Km for NAD(P)H (mean ± SD) (μM) | kcat (mean ± SD) (min−1) | kcat/Km for NAD(P)H (μM−1 min−1) | Km for xylose (mean ± SD) (mM) |
|---|---|---|---|---|
| NADPH | 1.8 ± 0.5 | 3,600 ± 100 | 2,000 | 34 ± 4 |
| NADH | 16 ± 4 | 310 ± 10 | 19 | 37 ± 7 |
All assays were performed at 25°C in 50 mM MOPS, pH 6.3.
