TABLE 2.
N. crassa XR with indicated substrate | kcat (mean ± SD) (min−1) | Km (mean ± SD) (mM) | kcat/Km (mM−1 min−1) | % Efficiency |
---|---|---|---|---|
d-Xylose | 3,600 ± 200 | 34 ± 4 | 110 | 100 |
d-Ribose | 3,120 ± 100 | 70 ± 10 | 45 | 41 |
l-Arabinose | 1,800 ± 100 | 40 ± 10 | 45 | 41 |
d-Galactose | 1,800 ± 100 | 180 ± 30 | 10 | 9.1 |
d-Glucose | 1,320 ± 100 | 360 ± 60 | 3 | 3.3 |
All assays were performed at 25°C in 50 mM MOPS, pH 6.3.