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Northern slot blot assays of sense strand in vitro rbcL transcription products probed with a digoxigenin-labeled fragment of rbcL from C. pelagicus. The upper luminograph shows the results obtained under suboptimal posthybridization wash conditions (0.2× rather than 0.05× SSPE in the stringency washes), while the lower luminograph shows the high specificity of the probe once the optimal hybridization protocol (see text) had been established.
