Fig. 2.

Animal experimental design and bodyweight of mice. (A) Six- (young) and 16-month (middle-aged) old mice were divided into five groups: P5, P15, P25, P35, and P45, which consumed diets with the same lipid content (25 kcal% fat) but different carbohydrate (70, 60, 50, 40, or 30 kcal% carbohydrate, respectively) and protein contents (5, 15, 25, 35, or 45 kcal% protein, respectively) as shown in Table 1. Also refer to Supplemental Fig. 2. (B) Representative appearance of mice after 8 weeks of feeding. (C) Body weight of young and middle-aged mice (n = 6–8 per group) at 8 weeks of feeding. Data are presented as mean ± SEM and analyzed using two-way ANOVA followed by Tukey post-test to examine the effects of diet (C). ^* p < 0.05, ^** p < 0.01