Fig. 7.

Plasma amino acid concentrations in young and middle-aged mice fed with different protein diets. Plasma concentrations of (A) leucine, (B) isoleucine, (C) valine, (D) phenylalanine, (E) lysine, (F) tryptophan, (G) threonine, (H) histidine, (I) methionine, (J) glycine, (K) cystine, (L) glutamine, (M) asparagine, (N) serine, (O) arginine, (P) alanine, (Q) proline, (R) tyrosine, (S) glutamic acid, (T) aspartic acid, (U) essential amino acid (EAA), non-essential amino acid (NEAA), (V) EAA, (W) NEAA, and (X) branched-chain amino acid (BCAA) in young and middle-aged mice (n = 6–7 per group) after 8 weeks of feeding. Data are presented as mean ± SEM and analyzed using two-way ANOVA followed by a Sidak post-test to examine the effect of age or Tukey post-test to examine the effects of diet (A–X). ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001